Hyperglycemia is a state of blood glucose levels above normal limits. Kiwifruit (Actinidia deliciosa) has a potential antidiabetic effects in lowering blood glucose levels. This research was aimed to determine the effectiveness of kiwi fruit (Actinidia deliciosa) extract to decrease blood glucose levels in male Wistar rats. This research used 25 white male rats as sample that were randomized used simple random sampling. Samples were divided into control positive group (Acarbose), control negative group (CMC), and kiwi fruit extract groups (500 mg/kg, 1.000 mg/kg, 1.500 mg/kg). The data were obtained every 30 minute, started from 30 minute after intervention till 2 hours after first examination. Data were analyzed with T-test and Kruskal-Wallis test. The result of T-test showed a significant difference of white male rats blood glucose levels (p <0.05). The result of Kruskal-Wallis test showed a significant difference in blood glucose levels of more than two groups (p <0.05). The Mann-Whitney test showed significant difference (p< 0.05) between kiwi fruit extract and control positive group with control negative group. The highest rank of mean difference was observed in 1.500 mg/kg (55.4 mg/dl) dose of kiwi fruit extract, the effect on decrease blood glucose levels in male Wistar rats was caused by flavonoid and phenolic compound that contained in kiwi fruit extract.